@ARTICLE{TreeBASE2Ref16964, author = {Myung Soo Park and Casey Culler and Barry M. Pryor}, title = {A re-examination of the phylogenetic relationship between the causal agents of carrot black rot, Alternaria radicina and A. carotiincultae}, year = {2007}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {The phylogenetic relationship between Alternaria radicina and A. carotiincultae was re-examined based on morphology, sequence analysis of rDNA [ITS and mitochondrial small subunit (mtSSU)], protein coding genes [actin (ACT), ??-tubulin, chitin synthase (CHS), translation elongation factor (EF-1a), Alt a1, and glyceraldehyde-3-phosphate dehydrogenase (gpd)], and RAPD and ISSR analysis of total genomic DNA. Although some morphological characters overlapped to a degree, with A. radicina isolates expressing moderate variation and A. carotiincultae isolates being highly uniform, A. carotiincultae could be differentiated from A. radicina based on significantly greater growth rate on potato dextrose agar (PDA) or acidified PDA (APDA) and average number of trasverse septa per conidium. Sequence of rDNA and two protein coding genes, ACT and CHS, were invariant between species. However, polymorphism with the EF-1a, ??-tubulin, and Alt a1 gene strictly separated the population of A. radicina and A. carotiincultae as distinct lineages, as did RAPD and ISSR analysis. The polymorphic gpd gene did not strictly separate the two species. However, isolates of A. radicina encompassed several haplotypes, one of which was the exclusive haplotype possessed by A. carotiincultae isolates, suggesting evidence of incomplete lineage sorting. The results suggest that A. carotiincultae is very closely related to A. radicina, but is a recently divergent and distinct lineage, which supports its status as a separate species.} }

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