@ARTICLE{TreeBASE2Ref2101, author = {Janusz Błaszkowski and G?bor M. Kov?cs and T?mea Bal?́zs}, title = {Glomus perpusillum, a new arbuscular mycorrhizal fungus.}, year = {2009}, keywords = {}, doi = {10.3852/08-087}, url = {}, pmid = {}, journal = {Mycologia}, volume = {101}, number = {2}, pages = {247--255}, abstract = {A new arbuscular mycorrhizal fungal species of the genus Glomus, G. perpusillum (Glomeromycota), forming small, hyaline spores is described and illustrated. Spores of G.perpusillum were formed in hypogeous aggregates and occasionally inside roots. They are 38 globose to subglobose, (10-)24(-30) ?m diam, rarely egg-shaped, oblong to irregular, 18-25 x 25-63 ?m. Their wall consists of two permanent layers: a finely laminate, semiflexible to rigid outer layer and a flexible to semiflexible inner layer. The inner layer becomes plastic and frequently highly contracts in spores vigorously crushed in PVLG-based mountants and stains reddish white to grayish red in Melzers reagent. Glomus perpusillum was originally associated with roots of Ammophila arenaria colonizing sandy dunes of the Mediterranean Sea adjacent to Calambrone (Italy) and this is the only site of its occurrence known to date. In one-species cultures with Plantago lanceolata as the host plant, G. perpusillum formed 46 vesicular-arbuscular mycorrhizae. Phylogenetic analyses of partial SSU sequences of nrDNA placed the species into the Glomus group A with no affinity to its subgroups. The sequences of G. perpusillum unambiguously separated from the sequences of described Glomus species and formed a distinct clade among with in planta arbuscular fungal sequences from Alpine plants.} }

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