@ARTICLE{TreeBASE2Ref18785, author = {Eri Hasegawa and Yuko Ota and Tsutomu Hattori and Taisei Kikuchi}, title = {Sequence-based identification of Japanese Armillaria species using the elongation factor-1 alpha gene.}, year = {2010}, keywords = {Armillaria, translation elongation factor-1 alpha gene, IGS, ITS, RFLP, species identification}, doi = {10.3852/09-238}, url = {}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {We analyzed the sequences of 3 DNA regions?the translation elongation factor-1 alpha (EF-1 alpha) gene and the internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of ribosomal DNA?to compare their accuracy in identifying different species of Japanese Armillaria. We studied 49 isolates of 8 Armillaria species: A. mellea, A. ostoyae, A. nabsnona, A. cepistipes, A. gallica, A. sinapina, A. tabescens and the biological species Nagasawa E (Nag. E). The phylogenetic analyses of the ITS and IGS data helped in identifying A. mellea, A. ostoyae, A. nabsnona, A. tabescens and Nag. E, but could not be used to identify A. gallica, A. cepistipes and A. sinapina. Nevertheless, our analysis showed that the EF-1 alpha gene was clearly different in the 8 examined species. Restriction fragment length polymorphisms (RFLPs) of the IGS-1 region could be used to distinguish most species, but the RFLP profiles of some isolates of A. cepistipes and A. sinapina were the same even with 4 different restriction enzymes. In conclusion, among the techniques examined in this study, analyzing the EF-1 alpha sequence was found to be the most suitable method for identifying the different species of Japanese Armillaria.} }

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