@ARTICLE{TreeBASE2Ref16250, author = {Steven R. Larson and John F. Doebley}, title = {Restriction site variation in the chloroplast genome of Tripsacum (Poaceae): Phylogeny and rates of sequence evolution.}, year = {1994}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Systematic Botany}, volume = {19}, number = {}, pages = {21--34}, abstract = {Restriction site variation was surveyed in the chloroplast genome (cpDNA) of 14 species of Tripsacum, two species of Zea, and an outgroup, Elionurus tripsacoides. A total of 783 cpDNA restriction sites was surveyed using 22 restriction endonucleases, revealing 110 polymorphic restriction sites among or within the species. We observed a relatively low level of cpDNA sequence divergence among Tripsacum species (0.0 to 0.0036 substitutions per site) as compared to other genera of flowering plants. The level of cpDNA sequence divergence between Zea and Tripsacum is also low (0.007 substitutions per site), suggesting that these genera are closely related. Variable restriction sites were used to construct parsimony phylogenetic trees. These trees failed to show close agreement with taxonomic relationships within Tripsacum. The trees do not show a division of Tripsacum into sects. Tripsacum and Fasciculata. Chloroplast DNA also indicates that T. dactyloides is not monophyletic. Comparison of cpDNA and rDNA restriction site variation suggests that interspecific introgressive hybridization could explain the low level of congruence between taxonomy and cpDNA. Tests of the molecular clock hypothesis indicate that cpDNA evolves more rapidly in Zea than in Tripsacum.} }

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