TreeBASE Search-Analyses for study 27370

@ARTICLE{TreeBASE2Ref31527, author = {Marin Talbot Brewer and Courtney J Cameron and Cynthia T Chan and Bhabesh Dutta and Ronald D. Gitaitis and L J Grauke and Jason H. Brock and Timothy B. Brenneman}, title = {Neofusicoccum caryigenum, a new species causing leaf die-back disease of pecan (Carya illinoinensis) }, year = {2021}, keywords = {Botryosphaeriaceae; cryptic species; hickory; Juglandaceae; plant pathogen}, doi = {}, url = {http://}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {Neofusicoccum species are endophytes and pathogens of woody hosts and members of the Botryosphaeriaceae. Leaf die-back is a new disease resulting in death of compound leaves and extensive defoliation of pecan trees (Carya illinoinensis) throughout the southeastern United States. Currently, the disease is primarily observed on trees that are not managed with fungicides for pecan scab. Preliminary observations of the fungus isolated from symptomatic leaves indicated it was a member of the genus Neofusicoccum. Our objectives were to confirm that this is the causal organism of leaf die-back disease of pecan and to determine if this disease is caused by a new or previously described species of Neofusicoccum. Morphological observations of pure cultures, conidiomata, conidiogenous cells, and conidia were consistent with members of the genus Neofusicoccum. Using Koch?s postulates, we established that Neofusicoccum sp. isolated from symptomatic leaves caused the disease. We sequenced the internal transcribed spacer of the rDNA (ITS), elongation factor 1-α (EF1-α), RNA polymerase subunit 2 (RPB2), and ?-tubulin (TUB2) of 11 isolates collected from Georgia and Texas. Phylogenetic and network analyses of these sequences combined with publicly available sequences of 40 members of the N. parvum - N. ribis species complex and the outgroup N. australe revealed that this fungus is a member of the species complex, but is genetically distinct from previously described species. We determined that leaf die-back of pecan is caused by a novel species, named herein N. caryigenum. } }