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Citation for Study 15820

Citation title: "Genetic polymorphism at an odorant receptor gene (Or39) among mosquitoes of the Anopheles gambiae complex in Senegal (West Africa)".

Study name: "Genetic polymorphism at an odorant receptor gene (Or39) among mosquitoes of the Anopheles gambiae complex in Senegal (West Africa)".

This study is part of submission 15820 (Status: Published).

Citation

Arnal A., Kengne P., Brengues C., Dabire R.K., Diabate A., Bassene H., & Simard F. 2014. Genetic polymorphism at an odorant receptor gene (Or39) among mosquitoes of the Anopheles gambiae complex in Senegal (West Africa). BioMed Central Research Note, .

Authors

• Arnal A. (submitter) 0467416147
• Kengne P.
• Brengues C.
• Dabire R.K.
• Diabate A.
• Bassene H.
• Simard F.

Abstract

Background Olfaction plays a significant role in insect behavior during critical steps of their life-cycle, such as host-seeking during foraging or the search for a mate. Here, we explored genetic polymorphism within and divergence between sibling species of the African malaria mosquito, Anopheles gambiae sensu lato in the gene sequence and encoded peptides of an odorant receptor, Or39. This study included sympatric specimens of An. gambiae sensu stricto, An. coluzzii and An. arabiensis sampled together in the village of Dielmo, Senegal. Results A 1,601bp genomic sequence composed of 6 exons and 5 introns was obtained for Or39 from 6-8 mosquitoes in each of the 3 species. DNA sequence analysis revealed a high level of molecular polymorphism (&#960; = 0.0154; Haplotype diversity = 0.867) and high overall genetic differentiation between taxa (Fst>0.92, P<0.01). In total, 50 parsimony informative sites were recorded. Throughout the whole dataset, there were 13 non-synonymous mutations resulting in aminoacid changes in the encoded protein. Each of the 6 different identified peptides was species-specific and none was shared across species. Most aminoacid changes were located on the intracellular domains of the protein. However, intraspecific polymorphisms in An. gambiae and An. arabiensis as well as species-specific mutations also occurred in the first extracellular domain. Conclusions Although obtained from a limited number of specimens, our results point towards genetic differences between cryptic species within the An. gambiae complex in a gene of biological relevance that might be of evolutionary significance when exposed to disruptive selective forces.

Keywords

Mosquito, malaria, Anopheles gambiae, speciation, olfactory receptor

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S15820
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref23209,
  	 author = {audrey  arnal and pierre  kengne and Cecile  Brengues and Roch Kounbobr Dabire and abdoulaye  diabate and Hubert  Bassene and Frederic  Simard},
  	 title = {Genetic polymorphism at an odorant receptor gene (Or39) among mosquitoes of the Anopheles gambiae complex in Senegal (West Africa)},
  	 year = {2014},
  	 keywords = {Mosquito, malaria, Anopheles gambiae, speciation, olfactory receptor},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {BioMed Central Research Note},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Background 
  Olfaction plays a significant role in insect behavior during critical steps of their life-cycle, such as host-seeking during foraging or the search for a mate. Here, we explored genetic polymorphism within and divergence between sibling species of the African malaria mosquito, Anopheles gambiae sensu lato in the gene sequence and encoded peptides of an odorant receptor, Or39. This study included sympatric specimens of An. gambiae sensu stricto, An. coluzzii and An. arabiensis sampled together in the village of Dielmo, Senegal.
  Results
  A 1,601bp genomic sequence composed of 6 exons and 5 introns was obtained for Or39 from 6-8 mosquitoes in each of the 3 species. DNA sequence analysis revealed a high level of molecular polymorphism (&#960; = 0.0154; Haplotype diversity = 0.867) and high overall genetic differentiation between taxa (Fst>0.92, P<0.01). In total, 50 parsimony informative sites were recorded. Throughout the whole dataset, there were 13 non-synonymous mutations resulting in aminoacid changes in the encoded protein. Each of the 6 different identified peptides was species-specific and none was shared across species. Most aminoacid changes were located on the intracellular domains of the protein. However, intraspecific polymorphisms in An. gambiae and An. arabiensis as well as species-specific mutations also occurred in the first extracellular domain.
  Conclusions
  Although obtained from a limited number of specimens, our results point towards genetic differences between cryptic species within the An. gambiae complex in a gene of biological relevance that might be of evolutionary significance when exposed to disruptive selective forces.
  }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 23209
  AU  - arnal,audrey 
  AU  - kengne,pierre 
  AU  - Brengues,Cecile 
  AU  - Dabire,Roch Kounbobr
  AU  - diabate,abdoulaye 
  AU  - Bassene,Hubert 
  AU  - Simard,Frederic 
  T1  - Genetic polymorphism at an odorant receptor gene (Or39) among mosquitoes of the Anopheles gambiae complex in Senegal (West Africa)
  PY  - 2014
  KW  - Mosquito
  KW  - malaria
  KW  - Anopheles gambiae
  KW  - speciation
  KW  - olfactory receptor
  UR  - http://dx.doi.org/
  N2  - Background 
  Olfaction plays a significant role in insect behavior during critical steps of their life-cycle, such as host-seeking during foraging or the search for a mate. Here, we explored genetic polymorphism within and divergence between sibling species of the African malaria mosquito, Anopheles gambiae sensu lato in the gene sequence and encoded peptides of an odorant receptor, Or39. This study included sympatric specimens of An. gambiae sensu stricto, An. coluzzii and An. arabiensis sampled together in the village of Dielmo, Senegal.
  Results
  A 1,601bp genomic sequence composed of 6 exons and 5 introns was obtained for Or39 from 6-8 mosquitoes in each of the 3 species. DNA sequence analysis revealed a high level of molecular polymorphism (&#960; = 0.0154; Haplotype diversity = 0.867) and high overall genetic differentiation between taxa (Fst>0.92, P<0.01). In total, 50 parsimony informative sites were recorded. Throughout the whole dataset, there were 13 non-synonymous mutations resulting in aminoacid changes in the encoded protein. Each of the 6 different identified peptides was species-specific and none was shared across species. Most aminoacid changes were located on the intracellular domains of the protein. However, intraspecific polymorphisms in An. gambiae and An. arabiensis as well as species-specific mutations also occurred in the first extracellular domain.
  Conclusions
  Although obtained from a limited number of specimens, our results point towards genetic differences between cryptic species within the An. gambiae complex in a gene of biological relevance that might be of evolutionary significance when exposed to disruptive selective forces.
  
  L3  - 
  JF  - BioMed Central Research Note
  VL  - 
  IS  - 
  ER  -