@ARTICLE{TreeBASE2Ref16574,
author = {P. G. Martin and J. M. Dowd},
title = {A comparison of 18s ribosomal RNA and rubisco large subunit sequences for studying angiosperm phylogeny.},
year = {1991},
keywords = {},
doi = {},
url = {},
pmid = {},
journal = {Journal of Molecular Evolution},
volume = {33},
number = {},
pages = {274--282},
abstract = {Partial sequences of 18s rRNA were obtained for 2 gymnosperms and 12 angiosperms from a wide range of families and these were analyzed with 5 other published sequences to form a phylogenetic tree. Using 16 published sequences of the large subunit of rubisco (rbcL), also from a wide range of angiosperm families, another phylogenetic tree was derived and the two approaches were compared. Both phylogenetic trees gave good grouping within families but in neither case was there resolution of the branching order of major taxa. Superficially the long rbcL sequences (whose base composition was homogeneous among all species) seemed very promising, but analysis showed that a large proportion of the variation did not affect the amino acid sequence. Although silent substitution contained some phylogenetic information, at the level required to order major taxa, much of it was random and obfuscating. It was concluded that neither macromolecule alone was likely to yield a solution to the problem of angiosperm phylogeny and therefore that studies of both, at least, will be required. For this reason, a method was described for obtaining both DNA and RNA of good quality from the same preparation and which had been used successfully with a wide range of species including many with pungent leaves.}
}
Citation for Study 175
Citation title:
"A comparison of 18s ribosomal RNA and rubisco large subunit sequences for studying angiosperm phylogeny.".
This study was previously identified under the legacy study ID S2x3x96c13c21c25
(Status: Published).
Citation
Martin P., & Dowd J. 1991. A comparison of 18s ribosomal RNA and rubisco large subunit sequences for studying angiosperm phylogeny. Journal of Molecular Evolution, 33: 274-282.
Authors
Abstract
Partial sequences of 18s rRNA were obtained for 2 gymnosperms and 12 angiosperms from a wide range of families and these were analyzed with 5 other published sequences to form a phylogenetic tree. Using 16 published sequences of the large subunit of rubisco (rbcL), also from a wide range of angiosperm families, another phylogenetic tree was derived and the two approaches were compared. Both phylogenetic trees gave good grouping within families but in neither case was there resolution of the branching order of major taxa. Superficially the long rbcL sequences (whose base composition was homogeneous among all species) seemed very promising, but analysis showed that a large proportion of the variation did not affect the amino acid sequence. Although silent substitution contained some phylogenetic information, at the level required to order major taxa, much of it was random and obfuscating. It was concluded that neither macromolecule alone was likely to yield a solution to the problem of angiosperm phylogeny and therefore that studies of both, at least, will be required. For this reason, a method was described for obtaining both DNA and RNA of good quality from the same preparation and which had been used successfully with a wide range of species including many with pungent leaves.
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S175
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- Show BibTeX reference
@ARTICLE{TreeBASE2Ref16574,
author = {P. G. Martin and J. M. Dowd},
title = {A comparison of 18s ribosomal RNA and rubisco large subunit sequences for studying angiosperm phylogeny.},
year = {1991},
keywords = {},
doi = {},
url = {},
pmid = {},
journal = {Journal of Molecular Evolution},
volume = {33},
number = {},
pages = {274--282},
abstract = {Partial sequences of 18s rRNA were obtained for 2 gymnosperms and 12 angiosperms from a wide range of families and these were analyzed with 5 other published sequences to form a phylogenetic tree. Using 16 published sequences of the large subunit of rubisco (rbcL), also from a wide range of angiosperm families, another phylogenetic tree was derived and the two approaches were compared. Both phylogenetic trees gave good grouping within families but in neither case was there resolution of the branching order of major taxa. Superficially the long rbcL sequences (whose base composition was homogeneous among all species) seemed very promising, but analysis showed that a large proportion of the variation did not affect the amino acid sequence. Although silent substitution contained some phylogenetic information, at the level required to order major taxa, much of it was random and obfuscating. It was concluded that neither macromolecule alone was likely to yield a solution to the problem of angiosperm phylogeny and therefore that studies of both, at least, will be required. For this reason, a method was described for obtaining both DNA and RNA of good quality from the same preparation and which had been used successfully with a wide range of species including many with pungent leaves.}
}
- Show RIS reference
TY - JOUR
ID - 16574
AU - Martin,P. G.
AU - Dowd,J. M.
T1 - A comparison of 18s ribosomal RNA and rubisco large subunit sequences for studying angiosperm phylogeny.
PY - 1991
UR -
N2 - Partial sequences of 18s rRNA were obtained for 2 gymnosperms and 12 angiosperms from a wide range of families and these were analyzed with 5 other published sequences to form a phylogenetic tree. Using 16 published sequences of the large subunit of rubisco (rbcL), also from a wide range of angiosperm families, another phylogenetic tree was derived and the two approaches were compared. Both phylogenetic trees gave good grouping within families but in neither case was there resolution of the branching order of major taxa. Superficially the long rbcL sequences (whose base composition was homogeneous among all species) seemed very promising, but analysis showed that a large proportion of the variation did not affect the amino acid sequence. Although silent substitution contained some phylogenetic information, at the level required to order major taxa, much of it was random and obfuscating. It was concluded that neither macromolecule alone was likely to yield a solution to the problem of angiosperm phylogeny and therefore that studies of both, at least, will be required. For this reason, a method was described for obtaining both DNA and RNA of good quality from the same preparation and which had been used successfully with a wide range of species including many with pungent leaves.
L3 -
JF - Journal of Molecular Evolution
VL - 33
IS -
SP - 274
EP - 282
ER -
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