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Citation for Study 19931

Citation title: "Microbiota of wild-caught Red Snapper Lutjanus campechanus ".

Study name: "Microbiota of wild-caught Red Snapper Lutjanus campechanus ".

This study is part of submission 19931 (Status: Published).

Citation

Tarnecki A.M., Patterson W.F., & Arias C.R. 2016. Microbiota of wild-caught Red Snapper Lutjanus campechanus. BMC Microbiology, .

Authors

• Tarnecki A.M. (submitter) 9413884441
• Patterson W.F.
• Arias C.R.

Abstract

Background: The microbiota plays an essential role in host health, particularly through competition with opportunistic pathogens. Changes in total bacterial load and microbiota structure can indicate early stages of disease, and information on the composition of bacterial communities is essential to understanding fish health. Although Red Snapper (Lutjanus campechanus) is an economically important species in recreational fisheries and a primary aquaculture candidate, no information is available on the microbial communities of this species. The aim of this study was to survey the microbiota of apparently healthy, wild-caught Red Snapper from the Gulf of Mexico. Sampled Red Snapper showed no physical signs of disease. Tissues that are either primary entry routes for pathogens (feces, gill) or essential to disease diagnosis (blood) were sampled. Bacteria were enumerated using culture-based techniques and characterized by pyrosequencing. Results: Aerobic counts of feces and gill samples were 107 and 104 CFU/g, respectively. All individuals had positive blood cultures with counts up to 23 CFU/g. Gammaproteobacteria dominated the microbiota of all sample types, including the genera Pseudoalteromonas and Photobacterium in feces and Pseudomonas in blood and gill. Gill samples were also dominated by Vibrio while blood samples had high abundances of Nevskia. High variability in microbiota composition was observed between individuals, with percent differences in community composition ranging from 6-76% in feces, 10-58% in gill, and 52-64% in blood. Conclusions: This study provides the first characterization of the microbiota of the economically significant Red Snapper via pyrosequencing. Its role in fish health highlights the importance of understanding microbiota composition for future work on disease prevention using microbial manipulation.

Keywords

Microbiota, Lutjanus campechanus, aquaculture, pyrosequencing

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S19931
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref26370,
  	 author = {Andrea Marie Tarnecki and William F Patterson and Covadonga R Arias},
  	 title = {Microbiota of wild-caught Red Snapper Lutjanus campechanus },
  	 year = {2016},
  	 keywords = {Microbiota, Lutjanus campechanus, aquaculture, pyrosequencing},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {BMC Microbiology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Background: The microbiota plays an essential role in host health, particularly through competition with opportunistic pathogens. Changes in total bacterial load and microbiota structure can indicate early stages of disease, and information on the composition of bacterial communities is essential to understanding fish health. Although Red Snapper (Lutjanus campechanus) is an economically important species in recreational fisheries and a primary aquaculture candidate, no information is available on the microbial communities of this species. The aim of this study was to survey the microbiota of apparently healthy, wild-caught Red Snapper from the Gulf of Mexico. Sampled Red Snapper showed no physical signs of disease. Tissues that are either primary entry routes for pathogens (feces, gill) or essential to disease diagnosis (blood) were sampled. Bacteria were enumerated using culture-based techniques and characterized by pyrosequencing. 
  Results: Aerobic counts of feces and gill samples were 107 and 104 CFU/g, respectively. All individuals had positive blood cultures with counts up to 23 CFU/g. Gammaproteobacteria dominated the microbiota of all sample types, including the genera Pseudoalteromonas and Photobacterium in feces and Pseudomonas in blood and gill. Gill samples were also dominated by Vibrio while blood samples had high abundances of Nevskia. High variability in microbiota composition was observed between individuals, with percent differences in community composition ranging from 6-76% in feces, 10-58% in gill, and 52-64% in blood. 
  Conclusions: This study provides the first characterization of the microbiota of the economically significant Red Snapper via pyrosequencing.  Its role in fish health highlights the importance of understanding microbiota composition for future work on disease prevention using microbial manipulation.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 26370
  AU  - Tarnecki,Andrea Marie
  AU  - Patterson,William F
  AU  - Arias,Covadonga R
  T1  - Microbiota of wild-caught Red Snapper Lutjanus campechanus 
  PY  - 2016
  KW  - Microbiota
  KW  - Lutjanus campechanus
  KW  - aquaculture
  KW  - pyrosequencing
  UR  - http://dx.doi.org/
  N2  - Background: The microbiota plays an essential role in host health, particularly through competition with opportunistic pathogens. Changes in total bacterial load and microbiota structure can indicate early stages of disease, and information on the composition of bacterial communities is essential to understanding fish health. Although Red Snapper (Lutjanus campechanus) is an economically important species in recreational fisheries and a primary aquaculture candidate, no information is available on the microbial communities of this species. The aim of this study was to survey the microbiota of apparently healthy, wild-caught Red Snapper from the Gulf of Mexico. Sampled Red Snapper showed no physical signs of disease. Tissues that are either primary entry routes for pathogens (feces, gill) or essential to disease diagnosis (blood) were sampled. Bacteria were enumerated using culture-based techniques and characterized by pyrosequencing. 
  Results: Aerobic counts of feces and gill samples were 107 and 104 CFU/g, respectively. All individuals had positive blood cultures with counts up to 23 CFU/g. Gammaproteobacteria dominated the microbiota of all sample types, including the genera Pseudoalteromonas and Photobacterium in feces and Pseudomonas in blood and gill. Gill samples were also dominated by Vibrio while blood samples had high abundances of Nevskia. High variability in microbiota composition was observed between individuals, with percent differences in community composition ranging from 6-76% in feces, 10-58% in gill, and 52-64% in blood. 
  Conclusions: This study provides the first characterization of the microbiota of the economically significant Red Snapper via pyrosequencing.  Its role in fish health highlights the importance of understanding microbiota composition for future work on disease prevention using microbial manipulation.
  L3  - 
  JF  - BMC Microbiology
  VL  - 
  IS  - 
  ER  -