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Citation for Study 25739

Citation title: "Genome size and endopolyploidy evolution across the moss phylogeny.".

Study name: "Genome size and endopolyploidy evolution across the moss phylogeny.".

This study is part of submission 25739 (Status: Published).

Citation

Bainard J.D., Newmaster S.G., & Budke J.M. 2020. Genome size and endopolyploidy evolution across the moss phylogeny. Annals of Botany, .

Authors

• Bainard J.D.
• Newmaster S.G.
• Budke J.M. (submitter) 8659746204

Abstract

Background and Aims Compared to other plant lineages, bryophytes have very small genomes with little variation across species, and high levels of endopolyploid nuclei. This study is the first analysis of moss genome evolution over a broad taxonomic sampling using phylogenetic comparative methods. We aim to determine whether genome size evolution is unidirectional as well as examine whether genome size and endopolyploidy are correlated in mosses. Methods Genome size and endoreduplication index (EI) estimates were newly generated using flow cytometry from moss samples collected in Canada. Phylogenetic relationships between moss species were reconstructed using GenBank sequence data and maximum likelihood methods. Additional 1C-values were compiled from the literature and genome size and EI were mapped onto the phylogeny to reconstruct ancestral character states, test for phylogenetic signal, and perform phylogenetic independent contrasts. Key Results Genome size and EI were obtained for over 50 moss taxa. New genome size estimates are reported for 33 moss species and new EIs are reported for 20 species. In combination with data from the literature, genome sizes were mapped onto a phylogeny for 173 moss species with this analysis indicating that genome size evolution in mosses does not appear to be unidirectional. Significant phylogenetic signal was detected for genome size when evaluated across the phylogeny, whereas phylogenetic signal was not detected for EI. Genome size and EI were not found to be significantly correlated when using phylogenetically corrected values. Conclusions Significant phylogenetic signal indicates closely related mosses have more similar genome sizes and EI values. This study supports that DNA content in mosses is defined by small genomes that are highly endopolyploid, suggesting strong selective pressure to maintain these features. Further research is needed to understand the functional significance of DNA content evolution in mosses.

Keywords

bryophytes, endopolyploidy, endoreduplication, flow cytometry, genome size, mosses, phylogenetic signal, phylogenetic independent contrasts (PIC)

External links

• DOI: 10.1093/aob/mcz194
• Other URL: http://doi.org/10.1093/aob/mcz194

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S25739
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref30423,
  	 author = {Jillian  D. Bainard and Steven G Newmaster and Jessica M.  Budke},
  	 title = {Genome size and endopolyploidy evolution across the moss phylogeny.},
  	 year = {2020},
  	 keywords = {bryophytes, endopolyploidy, endoreduplication, flow cytometry, genome size, mosses, phylogenetic signal, phylogenetic independent contrasts (PIC)},
  	 doi = {10.1093/aob/mcz194},
  	 url = {http://doi.org/10.1093/aob/mcz194},
  	 pmid = {},
  	 journal = {Annals of Botany},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Background and Aims Compared to other plant lineages, bryophytes have very small genomes with little variation across species, and high levels of endopolyploid nuclei. This study is the first analysis of moss genome evolution over a broad taxonomic sampling using phylogenetic comparative methods. We aim to determine whether genome size evolution is unidirectional as well as examine whether genome size and endopolyploidy are correlated in mosses. Methods Genome size and endoreduplication index (EI) estimates were newly generated using flow cytometry from moss samples collected in Canada. Phylogenetic relationships between moss species were reconstructed using GenBank sequence data and maximum likelihood methods. Additional 1C-values were compiled from the literature and genome size and EI were mapped onto the phylogeny to reconstruct ancestral character states, test for phylogenetic signal, and perform phylogenetic independent contrasts. Key Results Genome size and EI were obtained for over 50 moss taxa. New genome size estimates are reported for 33 moss species and new EIs are reported for 20 species. In combination with data from the literature, genome sizes were mapped onto a phylogeny for 173 moss species with this analysis indicating that genome size evolution in mosses does not appear to be unidirectional. Significant phylogenetic signal was detected for genome size when evaluated across the phylogeny, whereas phylogenetic signal was not detected for EI. Genome size and EI were not found to be significantly correlated when using phylogenetically corrected values. Conclusions Significant phylogenetic signal indicates closely related mosses have more similar genome sizes and EI values. This study supports that DNA content in mosses is defined by small genomes that are highly endopolyploid, suggesting strong selective pressure to maintain these features. Further research is needed to understand the functional significance of DNA content evolution in mosses.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 30423
  AU  - Bainard,Jillian  D.
  AU  - Newmaster,Steven G
  AU  - Budke,Jessica M. 
  T1  - Genome size and endopolyploidy evolution across the moss phylogeny.
  PY  - 2020
  KW  - bryophytes
  KW  - endopolyploidy
  KW  - endoreduplication
  KW  - flow cytometry
  KW  - genome size
  KW  - mosses
  KW  - phylogenetic signal
  KW  - phylogenetic independent contrasts (PIC)
  UR  - http://doi.org/10.1093/aob/mcz194
  N2  - Background and Aims Compared to other plant lineages, bryophytes have very small genomes with little variation across species, and high levels of endopolyploid nuclei. This study is the first analysis of moss genome evolution over a broad taxonomic sampling using phylogenetic comparative methods. We aim to determine whether genome size evolution is unidirectional as well as examine whether genome size and endopolyploidy are correlated in mosses. Methods Genome size and endoreduplication index (EI) estimates were newly generated using flow cytometry from moss samples collected in Canada. Phylogenetic relationships between moss species were reconstructed using GenBank sequence data and maximum likelihood methods. Additional 1C-values were compiled from the literature and genome size and EI were mapped onto the phylogeny to reconstruct ancestral character states, test for phylogenetic signal, and perform phylogenetic independent contrasts. Key Results Genome size and EI were obtained for over 50 moss taxa. New genome size estimates are reported for 33 moss species and new EIs are reported for 20 species. In combination with data from the literature, genome sizes were mapped onto a phylogeny for 173 moss species with this analysis indicating that genome size evolution in mosses does not appear to be unidirectional. Significant phylogenetic signal was detected for genome size when evaluated across the phylogeny, whereas phylogenetic signal was not detected for EI. Genome size and EI were not found to be significantly correlated when using phylogenetically corrected values. Conclusions Significant phylogenetic signal indicates closely related mosses have more similar genome sizes and EI values. This study supports that DNA content in mosses is defined by small genomes that are highly endopolyploid, suggesting strong selective pressure to maintain these features. Further research is needed to understand the functional significance of DNA content evolution in mosses.
  L3  - 10.1093/aob/mcz194
  JF  - Annals of Botany
  VL  - 
  IS  - 
  ER  -