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Citation for Study 27037

Citation title: "Coffee in Africa harbours a large diversity of Trichoderma: novel species, new records and their potential for biological control of coffee leaf rust ".

Study name: "Coffee in Africa harbours a large diversity of Trichoderma: novel species, new records and their potential for biological control of coffee leaf rust ".

This study is part of submission 27037 (Status: Published).

Citation

Rodriguez M.H., Evans H., Abreu L.M., Macedo D.M., Ndacnou M.K., & Bekele K.B. 2020. Coffee in Africa harbours a large diversity of Trichoderma: novel species, new records and their potential for biological control of coffee leaf rust. Scientific Reports, .

Authors

• Rodriguez M.H.
• Evans H.
• Abreu L.M. +55 31 98517 3859
• Macedo D.M. (submitter) 31988452409
• Ndacnou M.K.
• Bekele K.B.

Abstract

A survey for species of the genus Trichoderma occurring as endophytes of Coffea, and as mycoparasites of coffee rusts (Hemileia), was undertaken in Africa; concentrating on Cameroon and Ethiopia. Ninety-four isolates of Trichoderma were obtained during this study: 76 as endophytes of healthy leaves, stems and berries and, 18 directly from colonized rust pustules. A phylogenetic analysis of all isolates used a combination of three genes: translation elongation factor-1α (tef1), rpb2 and cal for selected isolates. GCPSR criteria were used for the recognition of species; supported by morphological and cultural characters. The results reveal a previously unrecorded diversity of Trichoderma species endophytic in both wild and cultivated Coffea, and mycoparasitic on Hemileia rusts. Sixteen species were delimited, including four novel taxa which are described herein: T. botryosum, T. caeruloviride, T. lentissimum and T. pseudopyramidale. Two of these new species, T. botryosum and T. pseudopyramidale, constituted over 60% of the total isolations, predominantly from wild C. arabica in Ethiopian cloud forest. In sharp contrast, not a single isolate of Trichoderma was obtained using the same isolation protocol during a survey of coffee in four Brazilian states, suggesting the existence of a ?Trichoderma void? in the endophyte mycobiota of coffee outside of Africa. The potential use of these African Trichoderma isolates in classical biological control, either as endophytic bodyguards ? to protect coffee plants from coffee leaf rust (CLR), caused by Hemileia vastatrix ? or to reduce the impact of CLR through mycoparasitism, is discussed, with reference to the on-going coffee-rust crisis in Central America.

Keywords

coffee, plant pathogen, fungi, biological control

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S27037
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref31295,
  	 author = {Maria del Carmen H Rodriguez and Harry C.  Evans and Lucas Magalhaes de Abreu and Davi Mesquita Macedo and Miraine Kapeua Ndacnou and Kifle Belachew Bekele},
  	 title = {Coffee in Africa harbours a large diversity of Trichoderma: novel species, new records and their potential for biological control of coffee leaf rust  },
  	 year = {2020},
  	 keywords = {coffee, plant pathogen, fungi, biological control},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Scientific Reports},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {A survey for species of the genus Trichoderma occurring as endophytes of Coffea, and as mycoparasites of coffee rusts (Hemileia), was undertaken in Africa; concentrating on Cameroon and Ethiopia. Ninety-four isolates of Trichoderma were obtained during this study: 76 as endophytes of healthy leaves, stems and berries and, 18 directly from colonized rust pustules. A phylogenetic analysis of all isolates used a combination of three genes: translation elongation factor-1α (tef1), rpb2 and cal for selected isolates. GCPSR criteria were used for the recognition of species; supported by morphological and cultural characters. The results reveal a previously unrecorded diversity of Trichoderma species endophytic in both wild and cultivated Coffea, and mycoparasitic on Hemileia rusts. Sixteen species were delimited, including four novel taxa which are described herein: T. botryosum, T. caeruloviride, T. lentissimum and T. pseudopyramidale. Two of these new species, T. botryosum and T. pseudopyramidale, constituted over 60% of the total isolations, predominantly from wild C. arabica in Ethiopian cloud forest. In sharp contrast, not a single isolate of Trichoderma was obtained using the same isolation protocol during a survey of coffee in four Brazilian states, suggesting the existence of a ?Trichoderma void? in the endophyte mycobiota of coffee outside of Africa. The potential use of these African Trichoderma isolates in classical biological control, either as endophytic bodyguards ? to protect coffee plants from coffee leaf rust (CLR), caused by Hemileia vastatrix ? or to reduce the impact of CLR through mycoparasitism, is discussed, with reference to the on-going coffee-rust crisis in Central America. }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 31295
  AU  - Rodriguez,Maria del Carmen H
  AU  - Evans,Harry C. 
  AU  - Abreu,Lucas Magalhaes de
  AU  - Macedo,Davi Mesquita
  AU  - Ndacnou,Miraine Kapeua
  AU  - Bekele,Kifle Belachew
  T1  - Coffee in Africa harbours a large diversity of Trichoderma: novel species, new records and their potential for biological control of coffee leaf rust  
  PY  - 2020
  KW  - coffee
  KW  - plant pathogen
  KW  - fungi
  KW  - biological control
  UR  - http://dx.doi.org/
  N2  - A survey for species of the genus Trichoderma occurring as endophytes of Coffea, and as mycoparasites of coffee rusts (Hemileia), was undertaken in Africa; concentrating on Cameroon and Ethiopia. Ninety-four isolates of Trichoderma were obtained during this study: 76 as endophytes of healthy leaves, stems and berries and, 18 directly from colonized rust pustules. A phylogenetic analysis of all isolates used a combination of three genes: translation elongation factor-1α (tef1), rpb2 and cal for selected isolates. GCPSR criteria were used for the recognition of species; supported by morphological and cultural characters. The results reveal a previously unrecorded diversity of Trichoderma species endophytic in both wild and cultivated Coffea, and mycoparasitic on Hemileia rusts. Sixteen species were delimited, including four novel taxa which are described herein: T. botryosum, T. caeruloviride, T. lentissimum and T. pseudopyramidale. Two of these new species, T. botryosum and T. pseudopyramidale, constituted over 60% of the total isolations, predominantly from wild C. arabica in Ethiopian cloud forest. In sharp contrast, not a single isolate of Trichoderma was obtained using the same isolation protocol during a survey of coffee in four Brazilian states, suggesting the existence of a ?Trichoderma void? in the endophyte mycobiota of coffee outside of Africa. The potential use of these African Trichoderma isolates in classical biological control, either as endophytic bodyguards ? to protect coffee plants from coffee leaf rust (CLR), caused by Hemileia vastatrix ? or to reduce the impact of CLR through mycoparasitism, is discussed, with reference to the on-going coffee-rust crisis in Central America. 
  L3  - 
  JF  - Scientific Reports
  VL  - 
  IS  - 
  ER  -