@ARTICLE{TreeBASE2Ref27656, author = {Anabela Lopes and Benedetto T Linaldeddu and Alan JL Phillips and Artur Alves}, title = {Mating type gene analyses in the genus Diplodia: from cryptic sex to cryptic species }, year = {2017}, keywords = {Botryosphaeriaceae, Heterothallism, PCR-based assay, Phylogenetic Informativeness}, doi = {}, url = {http://}, pmid = {}, journal = {Fungal Biology}, volume = {}, number = {}, pages = {}, abstract = {Cryptic species are common in Diplodia, a genus that includes some well-known and economically important plant pathogens. Thus, species delimitation has been based on the phylogenetic species recognition approach using multigene genealogies. We assessed the potential of mating type (MAT) genes sequences as phylogenetic markers for species delimitation in the genus Diplodia. A PCR-based mating type diagnostic assay was developed that allowed amplifying and sequencing MAT1-1-1 and MAT1-2-1 genes, and determining the mating strategies used by different species. All species tested were shown to be heterothallic. Phylogenetic analyses were performed on both MAT genes and also, for comparative purposes, on combined sequences of the ribosomal internal transcribed spacer (ITS), translation elongation factor 1-alpha (tef1-alpha) and beta-tubulin (tub2). MAT genes individual phylogenies clearly differentiated all species analysed and are in agreement with the results obtained with the commonly used multilocus phylogenetic analysis approach. However, MAT genes genealogies were superior to multigene genealogies in resolving closely related cryptic species. The phylogenetic informativeness of each locus was evaluated revealing that MAT genes were the most informative locus followed by tef1-alpha. Hence, MAT genes can be successfully used to establish species boundaries in the genus Diplodia.} }

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